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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in <t>neurotransmitter</t> levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.
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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in <t>neurotransmitter</t> levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.
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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in <t>neurotransmitter</t> levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.
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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in <t>neurotransmitter</t> levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.
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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in <t>neurotransmitter</t> levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.
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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in <t>neurotransmitter</t> levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.
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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in <t>neurotransmitter</t> levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.
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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in <t>neurotransmitter</t> levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.
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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in <t>neurotransmitter</t> levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.
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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in <t>neurotransmitter</t> levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.
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(A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in neurotransmitter levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.

Journal: Frontiers in Pharmacology

Article Title: Enhanced therapeutic potential of paeoniflorin and vitamin B12 in intracerebropeduncle ethidium bromide-induced multiple sclerosis-like pathology

doi: 10.3389/fphar.2026.1792674

Figure Lengend Snippet: (A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in neurotransmitter levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.

Article Snippet: Additionally, neurotransmitters like dopamine [KLR0219; Krishgen Biosystem, Mumbai, India], glutamate [KLR1474; Krishgen Biosystem, Mumbai, India], serotonin [KLR0866; Krishgen Biosystem, Mumbai, India], acetylcholine [KLR0722; Krishgen Biosystem, Mumbai, India] , and GABA [KLR0102; Krishgen Biosystem, Mumbai, India] were evaluated ( ).

Techniques: Standard Deviation, Control